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Denitrate removes nitrates, nitrites, ammonia, and organics from both fresh and marine water. The high porosity of denitrate supports the proliferation of both aerobic and anaerobic bacteria that further remove these toxic aquarium byproducts. Even when exhausted as an organic adsorbent, denitrate continues to be an excellent support for the biological filter and does not have to be removed. This product is sold by volume. Cited weight is minimal weight. Directions: For best results, denitrate should be placed to assure the Flow of water through it, such as in a canister filter, chemical filtration module, or box filter. Flow rate should not exceed 200 L (50 gallons) per hour. If higher Flow rates are unavoidable, use Matrix or Pond Matrix. It is best to rinse off dust before use. Once denitrate has been in use for several days, nitrate concentrations should start to fall and level off gradually at a concentration of about 4-5 mg/L as nitrate. As long as nitrate concentrations remain under control, the product is not exhausted. Each 1 L of denitrate treats about 200-400 L (50-100 gallons), depending on initial nitrate concentration and the current biological load. Enough should be used to remove nitrate at a rate at least as fast as the rate of formation. If very high nitrates are initially present, they should be brought down to less than 20 mg/L with water changes. 1 Liter / 34 fuel oz. (Treats 400 L (105 US Gallons)).