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One and Only Live Nitrifying Bacteria Eliminates new tank syndrome Instantly creates a biofilter Removes toxic ammonia and nitrite naturally Good for 6 months at room temperature 1 year at 50 degrees F. Best to keep refrigerated for longest shelf life. One and Only Live Nitrifying Bacteria prevents new tank syndrome by instantly working to establish a biofilter in newly set-up aquaria. Use One and Only to control ammonia and nitrite levels. One and Only Live Nitrifying Bacteria has no sulfur or other offensive odors. Directions for Use: SHAKE WELL BEFORE USING BEST TO USE THE ENTIRE CONTENTS AT ONE TIME Minimum dose 10 ml (2 capfuls) per 10 gallons of aquarium water. Cannot be overdosed. DO NOT FREEZE Use when setting up a new aquarium after water changes and disease treatments to quickly establish a natural biological filter.